<b>Spectrophotometric determination of yeast RNA with neutral red</b>

Dynamics of neutral lipid storage in yeast.

Since energy storage is a basic metabolic process, the synthesis of neutral lipids occurs in all kingdoms of life. The yeast, Saccharomyces cerevisiae, widely accepted as a model eukaryotic cell, contains two classes of neutral lipids, namely steryl esters and triacylglycerols. Triacylglycerols are synthesized through two pathways governed by the acyl-CoA diacylglycerol acyltransferase Dga1p an...

A Modified Orcinol Reaction for RNA Determination

The Determination of Neutral Fat Glycerol in Blood with Periodate

Recent work (1) on the blood precursors of milk fat, by attaching special significance to the triglycerides of blood in the formation of milk, has illustrated forcefully the need of a more direct method for the quantitative evaluation of neutral fat in blood. Until Kelsey (2) reported the use of lipases, the isolation of the glyceride fatty acids had not been accomplished because of the homolog...

Workflow for Genome-Wide Determination of Pre-mRNA Splicing Efficiency from Yeast RNA-seq Data

Pre-mRNA splicing represents an important regulatory layer of eukaryotic gene expression. In the simple budding yeast Saccharomyces cerevisiae, about one-third of all mRNA molecules undergo splicing, and splicing efficiency is tightly regulated, for example, during meiotic differentiation. S. cerevisiae features a streamlined, evolutionarily highly conserved splicing machinery and serves as a f...

Neutral Networks of Interacting RNA Secondary Structures

RNA molecules interact by forming inter-molecular base pairs that compete with the intramolecular base pairs of their secondary structures. Here we investigate the patterns of neutral mutations in RNAs whose function is the interaction with other RNAs, i.e., of co-folding RNAs. We find that (1) the degree of neutrality is much smaller in interacting RNAs compared to RNAs that just have to cofor...